Fig. 8 | Scientific Reports

Fig. 8

From: ANXA2 regulates mitochondrial function and cellular senescence of PDLCs via AKT/eNOS signaling pathway under high glucose conditions

Fig. 8

The effect of ANXA2/AKT/eNOS signaling pathway on Mitophagy of PDLCs under high glucose conditions. PDLCs were transfected with pcDNA3.1-ANXA2 or its empty vector controls, followed by treatment with high glucose, MK2206(10µM)(AKT inhibitor) and L-NAME (100µM)(eNOS inhibitor). (A) The expression of LC3, p62, Parkin at protein level in PDLCs. (B–D) Semi-quantitative analysis of LC3-II/I, p62, Parkin. All data are presented as the means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. CTRL: Control; 40G: 40mM glucose; 40G-A: 40mM glucose + ANXA2 overexpression. CTRL: Control; 40G: 40mM glucose; 40G-A: 40mM glucose + ANXA2 overexpression; 40G-A-MK: 40mM glucose + ANXA2 overexpression + MK2206(AKT inhibitor); 40G-A-LN: 40mM glucose + ANXA2 overexpression + L-NAME (eNOS inhibitor).

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