Fig. 4
From: NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy
Activation of general autophagy in the pilocarpine-induced epileptic rat model. (a) Western blot analysis of LC3B-II/I and p62 proteins in the hippocampus and temporal lobe cortex of the control group and epileptic (EP) rat group. The gels of different group (Hippocampus group and cortex group) were not cropped in the same gel. (b) Ralative RNA level of Beclin1, ATG5, ATG7, ATG12 in the hippocampus and temporal lobe cortex of the control group and epileptic (EP) rat group group (n = 3). (c) Immunofluorescence staining of LC3B and p62 in the hippocampus and temporal lobe cortex. Scale bar for fluorescence images: 100 μm. Unless specified otherwise, the data are presented as means ± SEM (error bar) and compared using the two-sided Student’s t test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
