Fig. 1
From: Establishment of enterotype-specific antibodies for various diagnostic systems

Reactivity and specificity of monoclonal antibodies (mAbs) established in this study based on ELISA. (A) ELISA of mAbs that were specifically reactive to heat-killed representative bacteria of human gut microbiome enterotypes Phocaeicola vulgatus JCM5826, Faecalibacterium duncaniae JCM31915, and Segatella copri JCM13464, but not to other gut bacteria, including Bifidobacterium pseudocatenulatum JCM1200, Bifidobacterium longum subsp. longum JCM1217, Blautia wexlerae JCM31267, and Akkermansia muciniphila JCM33894. Representative data for selected clones PV-L2B7-117K1, PV-S10F7-14K1, FD-S2D3-18K1, FD-L4F6-18K2, FD-L5B6-33K2, and SC-L10B5-35K1 are shown. (B) Comparison of the reactivity of established mAbs that were specifically reactive to representative bacteria of human gut microbiome enterotypes Faecalibacterium duncaniae JCM31915 and Segatella copri JCM13464, as detected by ELISA, to bacterial cells with ( +) or without (–) bead disruption. Representative data for selected clones FD-L5B6-33K2 and SC-L10B5-35K1 are shown. Data are the mean ± SD of two independent experiments. Statistical significance was determined using one-way ANOVA (A) and the Mann–Whitney U test (B). **P < 0.01, ***P < 0.001, and ****P < 0.0001. OD450, optical density at 450 nm.