Fig. 3 | Scientific Reports

Fig. 3

From: Establishment of enterotype-specific antibodies for various diagnostic systems

Fig. 3

Enhanced reactivity of SC-S10C3-49K1 monoclonal antibodies (mAbs) with access to cytoplasmic and cell-surface antigens. (A) Comparison of the reactivity of established mAbs that were specifically reactive to representative bacteria of human gut microbiome enterotypes Segatella copri JCM13464, as detected by ELISA, to bacterial cells with (+) or without (–) bead disruption. Representative data for selected clone SC-S10C3-49K1 are shown. Data are the mean ± SD of three independent experiments. Statistical significance was determined using the Mann–Whitney U test. OD450, optical density at 450 nm. (B) Reactivity of selected clones to representative bacteria of human gut microbiome enterotypes Phocaeicola vulgatus JCM5826, Faecalibacterium duncaniae JCM31915, and Segatella copri JCM13464, as determined by flow cytometry. Representative histograms are shown for P. vulgatus, F. duncaniae, and S. copri strains treated with (blue, green or red) or without (gray) selected clones. Data are representative of two independent experiments. FITC, fluorescein isothiocyanate.

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