Table 4 Mean values (± SE) of developmental period of different stages and other biological parameters of Phthorimaea absoluta after treatment with LC50 concentration of trans-anethole (TAL), Foeniculum vulgare pure essential oil (FVO), and FVO-loaded nanostructured lipid carrier (FVO-NLC) in comparison with control treatment.

From: Toxicity of Foeniculum vulgare essential oil, its main component and nanoformulation against Phthorimaea absoluta and the generalist predator Macrolophus pygmaeus

Biological parameters

Control*

TAL

FVO

FVO-NLC

Egg (days)

4.05 ± 0.05a

4.08 ± 0.03a

4.04 ± 0.05a

4.03 ± 0.04a

L1 (days)

3.14 ± 0.04a

3.07 ± 0.03a

3.12 ± 0.04a

3.12 ± 0.08a

L2 (days)

2.12 ± 0.04a

1.55 ± 0.05b

1.66 ± 0.06b

1.38 ± 0.06c

L3 (days)

2.92 ± 0.04c

3.82 ± 0.04b

3.68 ± 0.05b

4.21 ± 0.05a

L4 (days)

3.09 ± 0.05c

3.83 ± 0.06b

3.72 ± 0.07b

4.35 ± 0.07a

Pupa (days)

7.94 ± 0.06a

7.67 ± 0.06a

7.90 ± 0.07a

7.95 ± 0.09a

Egg-pupa (days)

23.24 ± 0.13c

24.02 ± 0.15b

24.14 ± 0.15b

25.11 ± 0.17a

Female longevity (days)

17.52 ± 0.09a

12.98 ± 0.02c

13.72 ± 0.10b

11.44 ± 0.09d

Male longevity (days)

15.79 ± 0.08a

11.00 ± 0.01c

12.05 ± 0.17b

10.21 ± 0.10d

Oviposition period (days)

12.02 ± 0.11a

6.08 ± 0.06c

8.21 ± 0.10b

5.31 ± 0.07d

Fecundity (eggs/female)

140.12 ± 1.13a

70.20 ± 0.34c

86.63 ± 1.21b

55.66 ± 0.67d

Egg-adult (days)

40.07 ± 0.19a

36.73 ± 0.17c

37.29 ± 0.19b

36.15 ± 0.21d

TPOP (days)

24.71 ± 0.16c

26.88 ± 0.18b

26.86 ± 0.20b

28.98 ± 0.22a

APOP (days)

1.45 ± 0.08d

3.02 ± 0.02b

2.70 ± 0.09c

3.76 ± 0.08a

  1. *=There was no significant difference between NLC sample prepared without FVO loading and distilled water-ethanol mixture, as controls. The means followed by different letters in each row are significantly different (paired bootstrap at 5% significance level). Standard errors were measured by 100,000 bootstrap resampling. L1-L4: larval instar. TPOP: total preovipositional period. APOP: adult preovipositional period.