Fig. 3
From: Automated image segmentation for accelerated nanoparticle characterization
To further separate the background from the foreground, we define a cutoff value by examining the pixel intensity of the upper-left-to-lower-right diagonal of the cropped image. The cropped image is shown in (a) with the diagonal overlaid in green for reference; (b) a plot of the pixel intensities along this diagonal, with the cutoff value as a red horizontal line; (c) a histogram of the difference in intensity values between the upper-left-to-lower-right and upper-right-to-lower-left diagonals across the entire dataset.
