Fig. 4
From: Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli
Subunit ratio of StxA2a and StxB2a in E. coli strains TS18/08, LB226692, HUSEC003, HUSEC004, HUSEC008, and HUSEC018. Subunit ratio was calculated from number of subunits of StxA2a and StxB2a, investigated under inducing growth conditions (100 ng/mL norfloxacin) using quantitative ELISA. Quantification was performed using a StxA2a and StxB2a standard within the range of 7.8 ng/mL and 1000 ng/mL. Data are means ± standard deviation of the experiments performed in three biological replicates (n = 3). Statical analysis was performed with the following tests: Shapiro–Wilk test, Tukey test and Bonferroni test. Significance in (b) indicates comparison to StxB2a:StxB2a ratio. Asterisks (**) indicate significance in subunit gene expression (p < 0.01).
