Table 1 Protein concentration, number of subunits of StxA2a and StxB2a, and subunit ratios in E. coli strains TS18/08, LB226692, HUSEC003, HUSEC004, HUSEC008, and HUSEC018.

From: Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli

Strain

protein concentration [µg/mL]*

number of subunits per mL [× 1012]*

subunit ratio [-]

StxA2a

StxB2a

StxA2a

StxB2a

StxA2a:StxB2a

TS18/08

0.91 ± 0.13

0.05 ± 0.00

17.06 ± 2.38

4.07 ± 0.02

4.19 ± 0.59

LB226692

0.96 ± 0.09

0.05 ± 0.00

17.99 ± 1.76

3.74 ± 0.29

4.19 ± 0.59

HUSEC003

1.29 ± 0.38

0.28 ± 0.01

24.19 ± 7.11

21.88 ± 0.82

1.12 ± 0.37

HUSEC004

0.49 ± 0.06

0.09 ± 0.01

9.27 ± 1.12

7.09 ± 0.72

1.34 ± 0.30

HUSEC008

0.33 ± 0.03

0.09 ± 0.01

6.27 ± 0.51

7.40 ± 0.46

0.85 ± 0.12

HUSEC018

1.58 ± 0.29

0.08 ± 0.02

29.79 ± 5.46

6.59 ± 1.52

4.82 ± 1.47

  1. *Protein concentration, number of subunits, and subunit ratios were investigated under inducing growth conditions (100 ng/mL norfloxacin) using quantitative ELISA. Quantification was performed using a StxA2a and StxB2a standard within the range of 7.8 ng/mL and 1000 ng/mL. Data are means ± standard deviation of the experiments performed in three biological replicates (n = 3).
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