Table 3 Oligonucleotides used in this study with respective PCR product size and PCR conditions.
From: Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli
oligonucleotide | nucleotide sequence (5′-3′ direction) | PCR product size | PCR conditions |
|---|---|---|---|
rrsB-for | GCATAACGTCGCAAGACCAAA | 91 bp | 95 °C, 10 s; 60.5 °C, 30 s; 72 °C, 10 s; 40 cycles |
rrsB-rev | GCCGTTACCCCACCTACTAGCT | ||
upper-stx | TCATATCTGGCGTTAATGGAGTTC | 99 bp | 95 °C, 10 s; 52.4 °C, 30 s; 72 °C, 10 s; 40 cycles |
lower-stx | GCGTAAGGCTTCTGCTGTG | ||
stxB2-for | TGAAGAAGATGTTTATGGCGG | 183 bp | 95 °C, 10 s; 55.8 °C, 30 s; 72 °C, 10 s; 40 cycles |
stxB2-rev | GAGCACTTTGCAGTAACGG |
