Fig. 3

The effects of the treated groups on the induction of apoptosis of NALM-6 cells with Annexin V-FITC/PI double staining. (A) Unstain, (B) Control, (C) Cytarabine: AraC (50 nM), (D) Metformin: Met (10 mM), (E) chloroquine: CQ (10 μM), (F) Met (20 mM)+AraC (15 nM), (G) CQ (10 μM)+AraC (10 nM), and (H) demonstrates apoptotic cells Percentage. Cell populations include early apoptotic (Annexin V+/PI-), late apoptotic (Annexin V+/PI +), and necrotic cells (Annexin V-/PI +). Data are represented as mean ± SD (n = 3 independent experiments).