Fig. 6
Genetic transfer in human cells, experiment programming and analysis of GFP protein expression. (A) Performing Cell Sowing, Jurkat Cells (Clone E6-1, TIB-152, ATCC) were cultivated in 4 wells at 2.5 × 104 cells/well. It was cultured in RPMI medium with a total volume of 500 µl. (B) Virus sample preparation, 1 ml of GFP (green fluorescent protein) Lentivirus was placed in one well of the 12-well plate. (C) Integration of Plates into the Autonomous Robot System, 12-well plates were placed in the frame of the Robot. (D) Addition of GFP Lentivirus to Cells, 70, 100, and 150° extractions were made from the well containing GFP Lentivirus (GFP LV) in a 12-well plate and added into wells 1, 2, and 3. (E) Incubation, after the robot performed the experiment, the 12-Well plate was placed in the incubator. (F) 70, 100, and 150° blocks were used for genetic transfer in human cells experiment programming. (G) 100°, and (H) 150° blocks that activate the pipette system are prepared in the experiment programming and perform the liquid withdrawal process. (I) Analysis of GFP protein expression in CRISPR.BOT modified transgenic Jurkat cell (Clone E6-1, TIB-152, ATCC) line using flow cytometry and (J) Fluorescence microscopy at 4X.
