Fig. 7
CRISPR gene modification and flow cytometry analysis. (A) Seeding of Jurkat Cells (Clone E6-1, TIB-152, ATCC) into 12 well plate at 5 × 104 cells/well. (B) virus sample preparation. gRNA 1,2,3 Lentivirus was placed in 12 well plate. (C) Integration of plates into the autonomous robot system, 12-well plates were placed in the frame of the Robot. (D) Addition of gRNA lentivirus to cells. The wells containing gRNA Lentivirus 1,2,3 in the 12-well plate were drawn at 100° and added to wells 1, 2, and 3 separately. (E) Incubation. After the robot performed the experiment, the plate was placed in the incubator. (F) flow cytometry analysis of GFP protein expression in transgenic human cells encoding CRISPR.BOT modified CRISPR guide RNAs. gRNA guide RNA, GFP green fluorescent protein.
