Fig. 3 | Scientific Reports

Fig. 3

From: Highly specific Immunoproteasome inhibitor M3258 induces proteotoxic stress and apoptosis in KMT2A::AFF1 driven acute lymphoblastic leukemia

Fig. 3

Specific inhibitors of the proteasome trypsin-like and the caspase-like sites enhance M3258 cytotoxicity. RS4;11 and SEM cells were treated with M3258 and indicated concentrations of the trypsin-like sites inhibitor LU-102 or the caspase-like sites inhibitor NC-021 for 48 h, and cell viability were measured with Alamar Blue. MLL-86 cells were treated ex vivo for 24 h, and cell viability was measured by the CellTiter Glo assay. All data are averages of two biological replicates. Error bars indicate the standard error. Single agent activity of NC-021 and LU-102 is presented on Supplementary Fig. S3 and in Fig. 3d in23. Bottom graph shows combination indexes determined by the CalcuSyn software.

Back to article page