Fig. 5
From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase
Frustration density distribution across Uricase structure. This visualization presents the uricase homotetramer in a ribbon cartoon format (gray), with frustration density mapped onto its structure. Highly frustrated interactions are marked by red dashed lines, while minimally frustrated interactions are represented by green dashed lines. Cysteine mutations are highlighted in yellow, indicating positions of potential disulfide bond formation aimed at enhancing structural stability. This structural frustration analysis elucidates the potential stabilization conferred by specific residue mutations. The visualization scripts were generated by frustratometer server at (available at http://frustratometer.qb.fcen.uba.ar/), the script then visualized using PyMOL version 3.0.0.
