Table 1 The evolutionary conserved cysteine pairs. Table shows the actual residues in modern Aspergillus flavus urate oxidase.

From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase

Residue pairs

D(Cα-Cα)*

D(Cβ-Cβ)*

Disulfide occurrence**

Disulfide frequency***

Ala 6—Cys 290

4.14

4.21

4

0.40

Ala 235—E 236

3.80

5.30

1

0.1

Gly 139—Ileu 140

3.78

N/A

1

0.1

  1. *Distances between two Cα and two Cβ.
  2. **Disulfide occurrence is a positive integer representing the number of times these positions are occupied by cysteines across homolog sequences.
  3. ***This value ranges from 0 to 100% and is obtained by dividing DOccur by the total number of proteins included in the multiple sequence alignment.
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