Table 4 The top cysteine mutation candidates, highlighting their stability effects and confidence levels.
From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase
Residue | ΔΔG | C_pred | Effect on stability | Confidence level |
|---|---|---|---|---|
D283 C | −0.052 | 0.884 | Slightly stabilizing | Very high |
N12 C | −0.114 | 0.864 | Mildly stabilizing | High |
S119 C | −0.202 | 0.841 | Mildly stabilizing | High |
A220 C | −0.102 | 0.839 | Slightly stabilizing | High |
A49 C | −0.062 | 0.857 | Slightly stabilizing | High |
A6 C | 0.096 | 0.842 | Destabilizing | High |
A225 C | 0.005 | 0.842 | Slightly destabilizing | High |
