Table 7 Pocket characteristic of uricase wild type and muteins.
From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase
Descriptor | Mutein-1 | Mutein-2 | Mutein-3 | Mutein-4 | Mutein-5 | Mutein-6 | WT |
|---|---|---|---|---|---|---|---|
Volume [Å3] | 293.376 | 301.568 | 303.104 | 305.664 | 298.496 | 290.304 | 299.520 |
Surface [Å2] | 393.967 | 413.211 | 413.211 | 411.622 | 401.297 | 400.819 | 442.963 |
Depth [Å] | 16.159 | 16.159 | 16.159 | 16.159 | 16.159 | 16.120 | 15.430 |
Enclosure | 0.947 | 0.949 | 0.949 | 0.955 | 0.952 | 0.948 | 0.921 |
Pocket atoms | 116 | 117 | 117 | 115 | 117 | 111 | 105 |
Hydrogen bond donors | 12 | 13 | 13 | 12 | 13 | 13 | 13 |
Hydrogen bond acceptors | 10 | 11 | 11 | 11 | 11 | 11 | 11 |
Hydrophobicity ratio | 0.707 | 0.692 | 0.692 | 0.696 | 0.692 | 0.694 | 0.686 |
Apolar amino acids | 11 | 11 | 11 | 11 | 11 | 11 | 11 |
Polar amino acids | 10 | 11 | 11 | 10 | 11 | 11 | 8 |
