Table 8 Frustration differences in comparison to wild type.

From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase

 

Highly frustrated difference

p-Value highly frustrated

Neutral frustrated difference

p-Value neutral frustrated

Minimally frustrated difference

p-Value minimally frustrated

Mutein-1

−0.017477

0.051981

−0.12481

1.25E-07

0.142287

2.89E-06

Mutein-2

−0.059387

0.000014

−0.122658

5.57E-06

0.182044

2.01E-06

Mutein-3

−0.17668

0.000008

−0.044691

1.31E-01

0.221371

1.13E-10

Mutein-4

−0.052436

0.000188

−0.059812

2.45E-02

0.112248

1.71E-03

Mutein-5

−0.142688

0.002567

−0.012536

5.85E-01

0.155224

2.18E-05

Mutein-6

−0.14926

0.000303

−0.036766

1.75E-01

0.186026

6.11E-06

  1. (A negative value indicates that the mutein has lower highly frustrated density compared to the wild type, suggesting improved stability. Similar interpretation applies for neutrally and minimally frustrated densities); positive values in these columns imply that the mutein maintains or increases stability in those categories compared to the wild type.A p-value less than 0.05 typically suggests that the difference is statistically significant.
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