Table 9 The energetic states of the tunnel to the active site of the enzyme.
From: Stability and functional consequences of disulfide bond engineering in Aspergillus flavus uricase
WT | Mutein 1 | Mutein 2 | Mutein 3 | Mutein 4 | Mutein 5 | Mutein 6 | |
|---|---|---|---|---|---|---|---|
E-bound | −4 | −4.2 | −5.3 | −4.1 | −4.1 | 13.1 | −6.3 |
E-max | −3.8 | −4.1 | −5.1 | −3.8 | −3.7 | 13.1 | −4.5 |
E-surface | −4.7 | −4.7 | −5.6 | −4.6 | −4.6 | −4.9 | −4.5 |
Ea | 0.9 | 0.6 | 0.5 | 0.8 | 0.9 | 18 | 0 |
ΔEBS | 0.7 | 0.5 | 0.3 | 0.5 | 0.5 | 18 | −1.8 |
