Fig. 2

Analysis of immune cell populations after VCA in the context of acute and chronic rejection. Activated B cells, plasma cells, and CD4 effector memory T cells increase significantly in the chronic rejection models. A, The composition of B cell population (CD45⁺CD3^-B220⁺) was analyzed in the BM and spleen at week 4 for the syngeneic, at week 1 for the acute, at week 2 for the chronic KO, and at week 6 for the chronic BM12 rejection model after VCA. And activated B cell (CD45⁺CD3^-B220⁺CD19⁺MHC II⁺CD138^-) and plasma cell (CD45⁺CD3^-B220⁺CD19⁺MHC II⁺CD138⁺) were analyzed in the spleen and BM respectively. B, The compositions of CD4 and CD8 T cell populations were investigated in the BM and spleen under different settings. C, CD4 T cells were subclassified depending on the status of CD44 and CD62L positivity such as central memory (CM; CD44⁺CD62L⁺) and effector memory (EM; CD44⁺CD62L^-) in the BM and spleen. Pooled data from 3 independent experiments. n = 6, ns; not significant, *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test, error bar; standard error of the mean.