Fig. 3

Increases in the levels of donor specific antigen (DSA) and C4 d in the systemic circulation of chronic rejection model recipients. A, A rapid ex-vivo incubation was performed between a donor’s (BALB/c or C57BL/6 backgrounds) lymphocytes and a recipient’s plasma. The plasma was collected after 4 weeks for the syngeneic, 1 week for acute, 2 weeks for chronic KO, and 6 weeks for chronic BM12 rejection groups after VCA. After 1 h incubation at 37 °C, Ig G⁺ cells on lymphocytes were analyzed by flow cytometry. n = 6, ***P < 0.001 by Student’s t test, error bar; standard error of the mean. B, C4 d levels were measured by ELISA using recipients’ plasma. Representative data from 3 independent experiments. n = 6, ns; not significant, *P < 0.05 by Student’s t test, error bar; standard error of the mean.