Fig. 3 | Scientific Reports

Fig. 3

From: Integrated proteomics highlights functional activation induced by advanced-platelet rich fibrin plus (A-PRF +) in primary equine fibroblasts

Fig. 3

A-PRF + improves cell function of primary equine fibroblasts. (A) Cell metabolism was examined by the MTT assay after 24 h of treatment. (B) Representative images of the clonogenic assay after 4 days. (C) Quantitative analysis of clonogenic potential obtained from 1% SDS cell dissolution and read to spectrophotometer. (D) Effect of A-PRF + on wound repair after a mechanical scratch. (E) Quantitative analysis expressed as wound area after 24 h. (F) Representative images of A-PRF + effect on cell cycle evaluated by propidium iodide assay via cytofluorimetric technique. (G) Quantitative analysis of DNA content. Results are expressed as mean ± standard deviation (SD) of three independent experiments. # denotes p < 0.05 versus 20% FBS; *, **, *** denote respectively p < 0.05, p < 0.01 and p < 0.001 versus Ctrl.

Back to article page