Fig. 6 | Scientific Reports

Fig. 6

From: Integrated proteomics highlights functional activation induced by advanced-platelet rich fibrin plus (A-PRF +) in primary equine fibroblasts

Fig. 6

A-PRF + increases collagen I production, cytokines, metalloproteases, growth factors gene expression and their release. (A) Confocal microscopy studies reveal collagen I production (green) after 24 h of treatment with A-PRF + . Cells are counterstained with phalloidin-tetra-methyl-rhodamine B isothiocyanate (red) and Hoechst 33,342 (blue) was used for nuclei. (B) Quantitative analysis of collagen I production. (C) Real-time PCR of EGF-R, FGF2 and TGF-β1 regenerative growth factors. (D) Real-time PCR of MT1-MMP, MMP2 and MMP9 metalloproteases. (E) Real-time PCR of IL-6, IL-8, IL-10 cytokines. (F) ELISA of PDGF-BB, VEGF-A and TGF-β1 regenerative growth factors. Scale bars (Fig. 6A: Ctrl, 20% FBS): 10 μm; Scale bar (Fig. 6A: A-PRF +): 5 μm. N ≥ 10. Results are expressed as mean ± standard deviation (SD) of three independent experiments. *, **, *** denote respectively p < 0.05, p < 0.01 and p < 0.001 versus Ctrl.

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