Fig. 1
Comparison of the Conventional-CE sequencer and the HiDy-CE sequencer. (A) Schematic comparison of binning methods in conventional-CE and HiDy-CE. On each of wavelength-dispersion images of fluorescences from four capillaries, 20 hardware-binning regions of 3 × 12 pixels are set for conventional-CE. Meanwhile, 240 hardware-binning regions of 3 × 1 pixels and 20 software-binning regions of 3 × 12 pixels are set for HiDy-CE. Each software-binning region for HiDy-CE can measure 12 times as much fluorescence as each hardware-binning region for conventional-CE. (B) Electropherograms overlay of KRAS G12R mutation (MT) analysis using commercially available DNA, prepared with STA, showing MT abundance ratios of 0 and 0.1%. Figures depict four replicate measurements per condition, with peak aligned for clarity. Upper-left panels shows result under standard protocol conditions (1.6-kV injection voltage) for conventional-CE (left) and HiDy-CE (right), demonstrating saturation of wild-type peaks. Lower-left panel illustrates HiDy-CE results with increased injection voltage (4.8 kV) to enhance detection of mutant peak.
