Fig. 3
Comparison of Conventional-CE and HiDy-CE for KRAS MT using pathological specimens. Left panels: Electropherograms analyzed using conventional-CE for KRAS MTs G12D, G12R, G12V, and G13D at VAFs of 0, 0.5, 1, and 5%. Four replicate measurements were conducted for each condition, with peaks aligned at WT peak top. Signal intensities were normalized so maximum intensity of WT peak is 1. WT peaks display saturated due to narrow dynamic range. Middle panels: Electropherograms using HiDy-CE under same conditions as above. Eight replicates were conducted, with peak alignments and intensity normalized like left panels. Wider dynamic range of HiDy-CE prevents WT peak saturation, enabling accurate VAF calculation. Right panels: Calibration curves for each MT type with HiDy-CE, showing SDs. X-axis represents prepared VAF, while Y-axis shows calculated VAF based on protocol illustrated in Suppl Fig. 1. Baseline VAF (0% input) is used as reference point.
