Fig. 5
Detection limits for KRAS MTs in diluted EUS-FNB samples. Diagrams examining detection thresholds for KRAS G12D, G12R, and G12V in EUS-FNB-derived DNA diluted with commercially available DNA. Triplicate measurements were conducted for each dilution series, plotting VAF of dPCR against that of HiDy-CE on X- and Y-axes, respectively. In dPCR measurements, if the number of MT droplets exceeds three, it is judged as harboring mutation. If the number of MT droplet is three or less, the VAF of dPCR is plotted as reference value. Detection limits were established where triplicate data consistently exceeded thresholds from clinical samples (see Suppl Fig. 2, right panels), determining detection limits at 2.1% for KRAS G12D, 3.1% for G12R, and 0.6% for G12V when diluting EUS-FNB sample with commercially available DNA. Thresholds of VAF were calculated from thresholds those of MT/WT (see Suppl Fig. 2, right panels) and calibration curves (see Fig. 3, right panels). Calibration curves are established from clinical samples (see Fig. 3, right panels).
