Table 1 Mutation detection capability on HiDy-CE using FNB samples.

From: High dynamic range capillary electrophoresis method for sensitive detection of low-frequency driver mutations

Patient

Pathologic diagnosis by FNB

DNA yield Qubit (ng)

10 ng

2 ng

HiDy

Targeted amplicon sequencing

HiDy

digital PCR

KRAS mutation

VAF (%)

KRAS

mutation

VAF (%)

KRAS

mutation

VAF (%)

KRAS

mutation

VAF (%)

1

Adenocarcinoma

1122

G12D

9.4

G12D

12.9

G12D

7.8

G12D

11.4

2

Adenocarcinoma

762

G12V*

27.4

G12V

26.6

G12V*

27.9

G12V

24.4

3

Adenocarcinoma

2388

G12R*

13.3

G12R

13.6

G12R

13.0

G12R

12.3

4

Atypical glands (dysplasia)

21

N/A

–

N/A

–

ND

 

ND

 

5

Adenocarcinoma

466

G12D

9.8

G12D

18.1

G12D

10.8

G12D

15.6

6

Adenocarcinoma

535

G12V*

48.9

G12V

46.2

G12V*

51.0

G12V

40.5

7

Adenocarcinoma

840

G12D

10.1

G12D

12.9

G12D

10.2

G12D

12.7

8

Adenocarcinoma

2940

G12V

5.3

G12V

7.3

G12V

6.7

G12V

4.1

9

Chronic pancreatitis

354

ND

 

ND

 

ND

 

ND

 

10

Duodenum mucosa

27

N/A

–

N/A

–

ND

 

ND

 

11

Adenocarcinoma

226

G12D

39.5

G12D

56.5

G12D*

39.0

G12D

46.3

12

Adenocarcinoma

1638

ND

 

ND

 

ND

 

ND

 

13

Adenocarcinoma

654

G12D

22.2

G12D

31.3

G12D

22.4

G12D

28.0

14

Adenocarcinoma

451

G12V

7.1

G12V

7.6

G12V

6.5

G12V

5.9

15

B cell lymphoma

882

ND

 

ND

 

ND

 

ND

 

16

GIST

346

ND

 

ND

 

ND

 

ND

 

17

GIST

179

*

*

ND

 

*

*

ND

 

18

Adenocarcinoma

139

G12V*

27.0

G12V

26.8

G12V*

28.1

G12V

23.8

19

GIST

298

ND

 

ND

 

ND

 

ND

 

20

Degeneration tissue

126

ND

 

ND

 

ND

 

ND

 

21

Adenocarcinoma

74

G12V*

34.4

G12V

31.6

G12V*

35.2

G12V

29.4

G12R

9.1

G12R

7.9

G12R

10.8

G12R

6.1

22

Adenocarcinoma

798

ND

–

ND

 

ND

 

ND

 

23

IOPN (high-grade dysplasia)

1320

ND

 

ND

 

ND

 

ND

 

24

Adenocarcinoma

210

G12V*

32.4

G12V

26.5

G12V*

32.0

G12V

24.7

G12R

16.8

G12R

11.7

G12R*

15.3

G12R

10.9

25

Atypical glands (dysplasia)

164

G12R*

12.1

G12R

12.3

G12R

11.0

G12R

9.5

26

Adenocarcinoma

85

G12D

20.5

N/A

–

G12D

22.3

G12D

27.6

27

No evidence of malignancy

22

N/A

–

N/A

–

ND

 

ND

 

28

Insufficient material

25

N/A

–

N/A

–

G12D

5.3

G12D

4.1

29

Insufficient material

39

N/A

–

N/A

–

G12D

3.3

G12D

6.4

30

Insufficient material

36

N/A

–

N/A

–

ND

 

ND

 

31

No evidence of malignancy

432

N/A

–

N/A

–

ND

 

ND

 

32

No evidence of malignancy

179

N/A

–

N/A

–

ND

 

ND

 

33

No evidence of malignancy

726

N/A

–

N/A

–

ND

 

ND

 

34

No evidence of malignancy

180

N/A

–

N/A

–

*

*

ND

 
  1. VAF Variant Allele Frequency, N/A not analyzed, ND not detected; *, including mutation detected at HiDy-CE but not detected at amplicon-targeted sequencing or digital PCR; GIST, gastrointestinal stromal tumor; IOPN, intraductal oncocytic –papillary neoplasm.
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