Fig. 4
From: Identification of SARS-CoV-2-binding lectins on a commercial lectin array
Binding of lectins to SARS-CoV-2 Spike glycoprotein. (a) SDS-PAGE of Spike glycoprotein (~ 140 KDa) expressed and purified from Expi293 F™ cells. (b) Western blot of purified Spike glycoprotein expressed and purified from Expi293 F™ cells. The blot was developed using mouse anti-His antibody and horseradish peroxidase-conjugated anti-mouse IgG. (c) Pseudo-coloured images showing the binding of control samples (bovine serum albumin (BSA) and phosphate buffered saline (PBS)) and different concentrations of purified Spike glycoprotein (5 µg, 10 µg, 10 µg, and 50 µg). The images were generated using the Protein Array Analyzer macro plugin in Image J. (d) Pseudo-coloured images showing the binding of four replicates (R1, R2, R3, and R4) of 10 µg purified Spike glycoprotein. The images were generated using the Protein Array Analyzer macro plugin in Image J. (e) An individual dot plot showing the average normalized percent relative fluorescence units (RFU) (normalized to positive and negative control) of binding of SARS-CoV-2 Spike glycoprotein to the 95 lectins in the lectin array. Average values were calculated from four lectin array runs (of 10 µg Spike glycoprotein). The lectins with visibly high binding are labelled. (f) Lectins that displayed > 100% RFU (after normalizing to positive and negative controls) for binding to SARS-CoV-2 Spike glycoprotein. (g, h) Normalized percent RFU of binding to the purified Spike glycoprotein for the lectins predicted to bind to N-core glycans (g) and O-core glycans (h). Green: Lectins predicted to bind to high mannose glycans; Orange: Lectins predicted to bind to complex as well as high mannose glycans; Yellow: Lectins predicted to bind to complex glycans; Red: Lectins predicted to bind to core-fucosylated glycans.
