Fig. 6
From: Identification of SARS-CoV-2-binding lectins on a commercial lectin array
Binding of lectins to SARS-CoV-2 cultured virus: (a) Pseudo-coloured images showing the binding of three replicates of SARS-CoV-2 enriched with PEG, and one control (culture supernatant from uninfected Vero E6 cells processed similarly). The images were generated using the Protein Array Analyzer macro plugin in Image J. (b) Pseudo-coloured images showing the binding of three replicates of SARS-CoV-2 enriched with Dynabead virus enrichment kit, and one control (culture supernatant from uninfected Vero E6 cells processed similarly). The images were generated using the Protein Array Analyzer macro plugin in Image J. (c) An individual dot plot showing the average normalized percent relative fluorescence units (RFU) (normalized to positive and negative control) of binding of SARS-CoV-2 enriched with PEG (in black color) and Dynabead virus enrichment kit (in red color) to the 95 lectins in the lectin array. Average values were calculated from three lectin array runs. The lectins with visibly high binding are labeled. (d) Lectins that bound with intensities greater than the positive control spots. The lectins in purple were identified in viruses enriched by PEG and Dynabead virus enrichment kit, and the lectins in black were identified only in viruses enriched by PEG. (e) Lectins with > fivefold differences in binding (p < 0.05, two-tailed paired t-test) to virus enriched by PEG versus that enriched by Dynabead virus enrichment kit. (f,g) Normalized percent RFU of binding to SARS-CoV-2 for the lectins predicted to bind to N-core glycans (f) and O-core glycans (g). Green: Lectins predicted to bind to high mannose glycans; Orange: Lectins predicted to bind to complex as well as high mannose glycans; Yellow: Lectins predicted to bind to complex glycans; Red: Lectins predicted to bind to core-fucosylated glycans.
