Fig. 3
From: CLN3 disease disrupts very early postnatal hippocampal maturation
CLN3 disease hippocampus is initially hyperexcitable then hypoexcitable to Schaffer collateral (SC) stimulation. (A) The VSDI response after SC stimulation of (B) p7, (C) p14, and (D) p21 Cln3−/− and Cln3+/+ hippocampus is shown. Raster plots of average fluorescence change (DF/F, warm colors excitation, cool colors inhibition) over time (x axis) and location within hippocampus (y axis) are shown. Stimulation results in both forward and backward propagation of excitation. At p7 and p14 there is a trend toward hyperexcitability in Cln3−/− hippocampus, but by p21, both forward and backward propagation of excitation is decreased. For each age group, Cln3−/− vs. Cln3+/+ rasters were compared using a permutation sampling method with 1000 iterations. Regions of significant (p < 0.05) hypoexcitability are shown in purple. N = at least 10 slices from 5 animals per condition. Location of stimulus shown by an asterix.
