Fig. 4

p30 Cln3 disease hippocampus shows background slowing with an increased delta / alpha ratio on EEG. (A) Long-term in vivo EEG recordings from the CA1 region of the hippocampus from p30 mice shows no significant difference in (B) interictal spikes or (C) total raw power between Cln3+/+ (black) and Cln3−/− (blue) hippocampus. However, there is significant background slowing in Cln3−/− hippocampus as indicated by a (D) increase in slow delta band (0.1–4 Hz) power, a (E) decrease in alpha band (8–12 Hz) power, and a resulting (F) increased delta: alpha ratio. Data were analyzed via 2-way ANOVA, with p-value for genotype as a source of variation shown, *p < = 0.05, **p < = 0.01. N = 7 Cln3+/+ and 6 Cln3−/− mice. Spikes were quantified as spikes / 30 min period averaged from 24 h of recording. Average power in each frequency band was calculated for 12 h of daytime and 12 h of night recordings from electrodes implanted into the right and left hippocampi.