Fig. 6
From: Limocitrin induced cellular death through ERK pathways in human oral squamous cell cancer
Role of XIAP in limocitrin -induced apoptosis activation. (A) Reduced XIAP expression observed in SCC-9 and SCC-47 cells after 24 h of limocitrin treatment. (B) Quantitative analysis of XIAP, cIAP-1, p-HSP27 and HSP27 levels in SCC-9 and SCC-47 cell lines. (C, F) Cotreatment of SCC-9 with siRNA negative control (si-NC), XIAP siRNA (si-XIAP), and limocitrin. Quantitation of cleaved PARP, caspase-3, and caspase-8 is illustrated. (D, G) Cotreatment of SCC-9 with cIAP-1 siRNA (si-cIAP1) and limocitrin. Quantitation of cleaved PARP, caspase-3, and caspase-8 is illustrated. (E, H) Cotreatment of SCC-9 with HSP27 siRNA (si-HSP27) and limocitrin. Quantitation of cleaved PARP, caspase-3, and caspase-8 is illustrated. All data are displayed as mean ± standard deviation (n = 3). *p < 0.05, indicating statistical significance compared with the control group. #p < 0.05, indicating statistical significance compared with limocitrin treatment alone.
