Fig. 5

Flow cytometry analysis of apoptosis in PANC-1 cells. Results showed a dose-dependent increase in apoptotic activity in PANC-1 cells following treatment with 4-FOC-conjugated NCC/CTAB/CS NPs. Apoptotic cell percentages for untreated controls were minimal (0.58% early, 1.62% late), while treatment at 31, 61, and 81 µg/mL significantly elevated early (3.34%, 6.15%, 5.48%) and late (23.9%, 44.1%, 54.1%) apoptosis rates, demonstrating effective induction of apoptosis.