Fig. 6

(A) Pearson’s Correlation Coefficients (PCC) for Tau-mCherry on LAMP1-positive DOs in Tau-GBA1^{L444P/L444P(I)}α fibroblasts treated for 4 days with the indicated GT-02216 concentration and overnight in the presence or absence of Tau seeds (mean ± sem, n = 100 cells). (B) As in A. for Tau-GBA1 ^WT(XX) fibroblasts. (C) Number of LAMP1-positive DOs per Tau-GBA1^{L444P/L444P(I)}α fibroblast treated as in A. (mean ± sem, n = 20 cells). A representative image of the three conditions (ctrl, Tau seeds, Tau seeds and 12.5 µmol/L GT-02216 are shown (Tau in magenta, LAMP1 one in yellow, nuclei stained with DAPI in blue). (D) As in C. for Tau-GBA1^WT(XX) fibroblasts. Ordinary 1way ANOVA (p < .0001, except for p < .01 in D.) and Dunnett’s multiple tests against the respective controls (light grey bars), * p < .05, **** p < .0001. (E) Number of LysoTracker-positive DOs per Tau-GBA1^{L444P/L444P(I)}α fibroblast treated as in A. (mean ± sem, n = 20 cells). (F) As in E. for Tau-GBA1^WT(XX) fibroblasts. Ordinary 1way ANOVA (p < .0001 for E; p 0.0024 for F) and Dunnett’s multiple tests against the respective controls (light grey bars), * p < .05, ** p < .01, **** p < .0001.