Fig. 6

Immunocytochemistry analysis of γH2AX foci formation in T98G cells in response to neutron irradiation in the presence or absence of 500 μM [¹⁰B]-BPA. When [¹⁰B]-BPA was present, an increase in γH2AX foci formation was observed following 1 min of neutron irradiation. At 10 min, many cells began to exhibit a pan-nuclear response, which was not present in the cells that received no treatment (NT). This demonstrates that neutron capture by [¹⁰B]-BPA increases the formation of DNA double-strand breaks and is significantly more pronounced than neutron irradiation alone. The pan-nuclear response observed at 10 min post-irradiation indicates that the damage is extensive and will likely result in cell death.