Fig. 3

Δred intensity influenced by (a) washing reagent, (b) methanol to ultrapure water percent ratio, (c) PNA1 and PNA2 concentration, (d) PNA1/dsDNA/PNA2 incubation time, (e) electrochemical detection step potential, (f) amplitude, and (g) frequency. The dsDNA concentration was 10 µM for experiments (c) and 4 µM for other experiments. The green bars indicate conditions chosen for subsequent experiments.