Fig. 2 | Scientific Reports

Fig. 2

From: Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy

Fig. 2

Polarization resolved SHG measurements on different structures. (A) PSHG images (red) recorded at polarization angle \(\phi =0^{\circ }\) of starch granule, human and mouse ECM, mouse muscle, alongside THG signals (green). (B) PSHG intensity profiles of a randomly selected pixel of interest. Color maps (C-F), frequency distributions (G-I), and mode values (J) of structure angle, \(\chi _{15}/\chi _{31}, \chi _{33}/\chi _{31}\), and \(\alpha\)-helix pitch angle for amylopectin, collagen fibers, and myofibrils. Same color represents same structure, one curve/dot represent one sample. * stands for p<0.05, ** for p<0.01, *** for p<0.001 and ns for no significant difference.

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