Fig. 1
From: Gut microbial ecology and function of a Pakistani cohort with Iron deficiency Anemia
Alpha diversity (Chao 1 Richness and Shannon entropy) comparison of (A) bacterial ASVs, as well as (B) KEGG KOs, and (C) MetaCyc pathways predicted from the PICRUSt2 software. Beta diversity (D-F) represented by principal coordinate analysis (PCoA) plots with each axis showing the percentage variability explained by that axis, and where ellipses represent 95% confidence interval of the standard error for a given group. We have used three different distance measures: (D) Bray-Curtis distance to show differences in composition, (E) Unweighted UniFrac distance to show differences in phylogeny, and (F) Hierarchical Meta-storms to show differences in metabolic function. PERMANOVA statistics utilising these distance measures are shown underneath the PCoA plots to suggest if there are significant differences between the groups with R2 value showing percentage variability explained. (G) shows the TSS + CLR normalized expressions of top 10 most abundant genera observed in this dataset. The solid lines in panels with boxplots connect groups if ANOVA is significant with significance values as: * p < 0.05, ** p < 0.01, or *** p < 0.001.
