Table 2 Redundancy analysis with both forward and reverse selection was performed to select the most important parameters that explain variation in the community matrices. The initial set of variables considered are as follows (with those selected in the final PERMANOVA models in bold case): status (Control; Anaemic), Mother’s HB, Miscarriage, Socio-economic status (Lower Middle Class; Middle Class; Upper Class), Weight, Diet, Demographic region (Rural; Urban). Here df, SS, and F are Degree of Freedom, Sum of Squared Errors, and F Statistic, respectively.

From: Gut microbial ecology and function of a Pakistani cohort with Iron deficiency Anemia

Covariates

df

SS

R2

F

P

Bray-Curtis Distance

Miscarriage

1

0.8378

0.0972

2.6917

0.015 *

Residual

25

7.7813

0.9028

  

Total

26

8.6191

1

  

Unweighted Uni-Frac

Demographic_region

1

0.4808

0.06365

1.6995

0.02 *

Residual

25

7.0721

0.93635

  

Total

26

7.5529

1

  

Weighted Uni-Frac

Miscarriage

1

0.49826

0.15895

5.4185

0.011 *

Socio_economic_status

2

0.41234

0.13154

2.2421

0.064.

Fish

1

0.04918

0.01569

0.5348

0.609

Baby_weight

1

0.24382

0.07778

2.6516

0.084.

Residual

21

1.93105

0.61603

  

Total

26

3.13465

1

  

Hierarchical Meta-Storms

Miscarriage

1

0.07190

0.11536

3.4033

0.027 *

Weight

1

0.04430

0.07108

2.0970

0.099.

Residual

24

0.50703

0.81355

  

Total

26

0.62323

1

  
  1. Significance codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘’ 1