Fig. 4

Putative new Cry toxin-like family based on structural homology. (A) The network of members of B. bassiana compared to Bt Cry toxin Cry51Aa1. Each orange node is similar to each other by calculating TM-score > 0.7 (Grey edge). In contrast, the green node represents the BBA_06207 protein (Blue node) with a high E-value (Red edge) structural similarity with Bt Cry toxin (Green node). (B) Structural similarity dendrogram between highly similar protein from other entomopathogenic fungi to BBA_09344 protein. The average DALI Z-score was 19.3 (cutoff Z = 2). (C) 3D structure comparison between Cry51Aa1 (PDB: 4PKM) and BBA_06207 (confidence score = 84.5). (D) Active site of BBA_06207 with threonine-rich residues.