Fig. 3
From: Early, very high-dose, and prolonged vitamin C administration in murine sepsis
Cecal ligation and puncture (CLP) promotes acute lung injury in mice. (A) Representative hematoxylin–eosin (H&E) staining of lungs euthanized 8 d after CLP (scale bar: 50 μm). (B) For quantification of lung injury, a histological score was evaluated based on H&E staining (n = 3–6 per group). Quantification of (C) occludin (OCLN; n = 3–5), (D) claudin-2 (CLDN2; n = 3–7), and (E) claudin-4 (CLDN4; n = 3–7) mRNA expression levels from lungs 8 d after CLP using quantitative real-time PCR. Data are shown as the mean ± standard error of the mean. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; one-way analysis of variance with Tukey’s multiple comparison test. ALI: acute lung injury; AscA: ascorbic acid; inj.: injection; ns: not significant.
