Fig. 4
From: Early, very high-dose, and prolonged vitamin C administration in murine sepsis
Cecal ligation and puncture (CLP) promotes acute kidney injury in mice. (A) Representative hematoxylin–eosin staining of kidneys euthanized 8 d after CLP (scale bar: 25 μm). (B) For quantification of kidney injury, a histological score was evaluated based on H&E staining (n = 6 per group). Quantification of (C) kidney injury molecule 1 (KIM1) and (D) neutrophil gelatinase-associated lipocalin (NGAL) mRNA expression levels from kidneys 8 d after CLP using quantitative real-time PCR (n = 2–3 per group). Data are shown as the mean ± standard error of the mean. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; one-way analysis of variance with Tukey’s multiple comparison test. AscA: ascorbic acid; inj.: injection; ns: not significant.
