Fig. 5
From: Early, very high-dose, and prolonged vitamin C administration in murine sepsis
Cecal ligation and puncture (CLP) promotes liver injury in mice. (A,B) Representative hematoxylin–eosin staining of liver euthanized 8 d after CLP (scale bar: 50 μm). (C) For quantification of liver injury, a histological score was evaluated based on H&E staining (n = 6 per group). (D) Representative western blot for cyclooxygenase-2 (Cox-2) and heme oxygenase 1 (HO-1; indicated by an arrow) from liver 8 d after CLP (n = 2 in the sham; n = 3 in the CLP; n = 3 in the CLP + AscA90; n = 3 in the CLP + AscA180; and n = 3 in the CLP + AscA360). (E) Quantitative graphs of the protein expression levels of Cox-2 and HO-1 compared to GAPDH (n = 4). Quantification of (F) prostaglandin-endoperoxide synthase 2 (PTGS2; n = 3–9) and (G) heme oxygenase 1 (HMOX1; n = 3–6) mRNA expression levels from liver 8 d after CLP using quantitative real-time PCR. Data are shown as the mean ± standard error of the mean. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; one-way analysis of variance with Tukey’s multiple comparison test. AscA: ascorbic acid; ns: not significant; Veh: vehicle.
