Fig. 8

LANCL2 promoted the proliferation of GC cells in vitro. (A) The expression of LANCL2 using qPCR and Wb. (B) The cell proliferation was measured using CCK8 asssay. (C) The cell proliferation was measured using colony formation assay. (D) The cell proliferation was measured using EDU assay. Red, proliferating cells; Blue, all cell nuclei; Percentage of positive stained cell (%) = Red cells/Blue cells (%); Scale bar, 50 μm. (E) The cell cycle was performed with flow cytometry. (F) Wb of cell cycle-related proteins in AGS and HGC-27. The cell experiments were replicated three times. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.