Table 3 Determination of relative cell viability (IC50 values) of cancer cell lines (colorectal cancer (DLD-1, HT-29, HCT-116), prostate cancer (PC-3) and pancreatic cancer (BxPC-3)) or normal cells: normal human colon tissue cells (CCD 841 CoN), human lung fibroblasts (WI-38 cells) and peripheral blood lymphocytes (PBLs), treated with MM124 and MM125 using 72-h MTT assay. The values were provided with standard deviation (± SD) [µM]. Additionally, median IC50 values obtained for MM-compounds in cancer and normal cells were summarized to calculate the selectivity index (SI) (ratio of median IC50 values in normal cell lines and cancer cell lines).
Compound | DLD-1 | HT-29 | HCT-116 | PC-3 | BxPC-3 | CCD 841 CoN | WI-38 | PBLs |
|---|---|---|---|---|---|---|---|---|
colorectal cancer | prostate cancer | pancreatic cancer | normal human colon tissue cells | normal human fibroblasts | peripheral blood lymphocytes | |||
MM124 | 0.49 ± 0.04 | 0.22 ± 0.02 | 0.38 ± 0.02 | 0.3 | 0.32 ± 0.04 | 1.12 ± 0.04 | 0.98 ± 0.13 | 0.87 ± 0.07 |
MM125 | 0.55 ± 0.13 | 0.27 ± 0.02 | 0.49 ± 0.08 | 0.43 ± 0.007 | 0.21 ± 0.02 | 1.15 ± 0.02 | 1.21 ± 0.03 | 1.109 ± 0.13 |
Median IC50 (with interquartile range) | 0.35 µM (0.26–0.49) | 1.11 µM (0.95–1.16) | ||||||
5-FU | 2.68 ± 1.35 | 4.38 ± 1.1 | 3.09 ± 0.92 | > 360 | 13.43 ± 1.9 | n/d | 42.18 ± 7.8 | n/d |
