Fig. 2
From: Far-ultraviolet light causes direct DNA damage in human lung cells and tissues
Pulsed 222 nm and 206 nm irradiation induced DNA DSBs damage in monolayered Beas-2B in vitro. (A) Representative immunofluorescence images of γH2AX(Ser139) staining (green) in Beas-2B cells 2 hours post-irradiation with pulsed 222 nm. Hoechst nuclei (blue). Pulsed 254 nm light (50 mJ/cm2) was used as a positive control of γH2AX(Ser139) staining. Scale bar, 100 μm. (B) Quantification of the γH2AX(Ser139) staining as the corrected total cell fluorescence (CTCF) was performed on Beas-2B cells 2 hours post-irradiation. Data presented as means +/-SD from n = 3 experiments. In total 237 cells were analyzed per condition. One-way ANOVA with repeated measures (****p < 0.0001). (C) Representative immunofluorescence images of γH2AX(Ser139) staining (green) in Beas-2B cells at 2 hours following pulsed 206 nm irradiation. Hoechst nuclei (blue). Scale bar, 100 μm. (D) Quantification of the γH2AX(Ser139) activation was performed on Beas-2B cells 2 hours post-irradiation with pulsed 206 nm. Data presented as means +/-SD from n = 3 experiments. In total 232 cells were analyzed per condition. One-way ANOVA with repeated measures (****p < 0.0001).
