Fig. 1 | Scientific Reports

Fig. 1

From: GDNF overexpression in astrocytes enhances branching and partially preserves hippocampal function in an Alzheimer’s rat model

Fig. 1

Hippocampal expression of the AAV9-(GFAP)-GDNF-TdTom vector. (A) Schematic of the experimental design to assess the expression of the vector and immunofluorescence images showing colocalization of TdTom in GFAP + astrocytes. (B) Left: pictures of a hippocampal coronal section DAB-stained for TdTom from an AAV injected animal. Scale bar at the left inset applies to the right one. Right: low magnification pictures of coronal brain sections DAB-stained for TdTom in animals injected in the hippocampus with the AAV9-(GFAP)-GDNF-TdTom. (C) Schematic of the experimental design for the qPCR study to assess GDNF levels in injected animals and the corresponding mean-annotated scatter plot. Data are referred to the GFP sample with lowest detection. N = 3 hippocampi/group. ** p < 0.01. (D) Left: picture of a hippocampal coronal section DAB-stained for GDNF from an AAV9-(GFAP)-GDNF-TdTom injected animal. Right: low magnification pictures of coronal brain sections DAB-stained for GDNF in the animal injected in the hippocampus with the AAV9-(GFAP)-GDNF-TdTom. SR: Stratum Radiatum. DH: Dentate Hilus.

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