Fig. 4
Microscopic analysis of the hippocampal astrocytes in the STZ experiment. (A) High magnification image of a representative astrocyte stained for GFAP from the SHAM group and cell reconstruction. (A’) Image of a representative cell from the STZ group. (A’’) Representative image of an astrocyte from the GFP + STZ group. The cell was identified with TdTom fluorescence and then reconstructed based on the morphology observed in the GFAP channel. (A’’’) A representative image of an astrocyte from the GDNF + STZ group. Scale in (A) applies to (A-A’’’). (B) Line plot showing group means displaying astrocyte intersections at different radii. Error bars represent 1 SEM. (C) Box plot with overlaid scatter plot showing the mean astrocyte process length for the animal groups. N = 8/group. *p < 0.05, **p < 0.01.
