Fig. 4

Effects of colchicine on localizations of insulin and Rab27A in MIN6 cells. (A) Localizations of insulin and V5-Rab27A in cells treated with colchicine. Cells expressing V5-Rab27A were preincubated with 2.2 mM glucose for 60 min, and then, in the absence (colchicine (–), upper panels) or presence (colchicine ( +), lower panels) of 2 μM colchicine, sequentially incubated with 2.2 mM glucose for 15 min, 20 mM glucose for 5 min, and 2.2 mM glucose for 5 min. The treated cells were stained with antibodies specific for insulin (magenta) and V5 (green). Merged images are also shown. (B) Effects of colchicine on the glucose-dependent peripheral localization of insulin. The percentage of cells with peripheral insulin was calculated in the absence (colchicine (–), open squares) and presence (colchicine ( +), closed squares) of 2 μM colchicine. Data are means ± S.E. from more than three independent experiments. n > 30 cells in each experiment. *p < 0.05, unpaired multiple t test (Holm-Šídák method).