Fig. 6
From: The a subunit isoforms of V-ATPase are involved in glucose-dependent trafficking of insulin granules
Interaction of a2 and a3 with EPI64, the GAP for Rab27A. FLAG-a2 or FLAG-a3 and V5-EPI64 were co-expressed in HEK293T cells. The cells were lysed, and lysates were immunoprecipitated with an anti-FLAG antibody. The precipitates were analyzed using antibodies against FLAG (top panel) and V5 (second panel from the top). In addition, 1% of the cell lysate was subjected to western blotting with an anti-V5 antibody (bottom two panels). Original blots are presented in Supplementary Fig. 11.
